本发明的重组抗原包括亚单位GluN1a重组蛋白，其氨基酸序列为将SEQ ID NO:1所示的序列中Asn61、Asn350、Asn471和Asn771突变为Gln，将Cys22突变为Ser，将Glu595和Glu597突变为Ser，将Glu598突变为Thr。该N‑甲基‑D‑天冬氨酸受体重组抗原还包括天然亚单位GluN2A重组蛋白和/或亚单位GluN2B重组蛋白，其氨基酸序列为将SEQ ID NO:3所示的序列中Asn348突变为Asp，Cys838和Cys849突变为Ser。
本发明公开一种多肽、IL17A/F单域抗体、核苷酸序列及试剂盒，其中，该多肽序列如下：Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Pro GlyGly Ser Leu Arg Leu Ser Cys Val Ala Pro Gly Asn Ile Phe Ser Asp Asn Ala Met Gly Trp Tyr Arg Gln AlaPro Gly Lys Gln Arg Glu Phe Val Ala His Ile Thr Thr Arg Ser Gly Ala Gly Tyr Val Asp Ser Val Lys Gly ArgPhe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met AsnSer Leu Lys Pro Glu Asp Thr AlaVal Tyr Tyr Cys Asn Thr Asn Pro Pro Met Trp Thr Tyr Trp Gly Gln Gly Thr Gln Val Thr Val SerSer。
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe SerSer Tyr Ala Met Gly Trp Tyr Arg Gln Ala ProGly Lys Glu Cys Glu Leu Val Ala Thr Ile Thr Ser Ala Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys GlyArg Phe Thr Leu Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met AsnSer Leu Lys Pro Glu Asp Thr AlaGlu Tyr Tyr Cys Asn Ala Glu SerSer Thr Thr Gly Trp Gly Thr Ile Cys Asp Ala Leu Asp Ala Phe Gly GlnGly Thr Gln Val Thr Val SerSer。
