[发明专利]华南雨蛙促皮肤损伤修复多肽及其基因和应用

权利要求书

1.华南雨蛙促皮肤损伤修复多肽，其特征在于该华南雨蛙促皮肤损伤修复多肽包括Hylareleasin3和Hylareleasin4，是中国两栖类动物华南雨蛙基因编码的直连多肽，分子量分别为1764.106道尔顿和1707.05道尔顿，等电点分别为9.61和9.60；华南雨蛙促皮肤损伤修复多肽Hylareleasin3和4都由16个氨基酸组成，全序列分别为NH₂-GLLDPVTHILGGFLRR-COOH和NH₂-GLLDPVTNILGGLLRR-COOH。

2.权利要求1所述的华南雨蛙促皮肤损伤修复多肽的基因核苷酸序列，其特征在于：cDNA由365个核苷酸组成，其自5’端至3’端序列为：

Hylareleasin3：

atgactttcttgaagaaatcacttttccttgtacgattccttggattagtttcactgtcc    60

atctgtgaagaaaagaaaagagaggaagaagaagaagagaaaagagatggtagtgaggag    120

gaagaagaggagaaaagagatgatagtgaggaagaagaagaggagaaaagagatgatgaa    180

aaagaggaagaaaatgaggcagatgcagaaaagaggggtttgctggaccctgtaacccat    240

atacttggaggttttcttcgcagacgtaaataaaatgtaatgtaataagaacagttacta    300

ttagttgtgccagatatatattaaagcatattcaaccaaaaaaaaaaaaaaaaaaaaaaa    360

aaaaa                                                           365

Hylareleasin4：

atgactttcttgaagaaatcacttttccttgtactattccttggattagtttcactgtcc    60

atctgtgaagaaaagaaaagagaggaagaagaagaagagaaaagagatggtagtgaggag    120

gaagaagaggagaaaagagatgatagtgaggaagaagaagaggagaaaagagatgatgaa    180

aaagaggaagaaaatgaggcagatgcagaaaagaggggtttgctggaccctgtaaccaat    240

atacttggaggtcttcttcgcagacgtaaataaaacgtaatgtaataagaacagttacta    300

ttagttgtgccagatatatattaaagcatattcaaccaaaaaaaaaaaaaaaaaaaaaaa    360

aaaaa                                                           365

3.编码权利要求2所述的华南雨蛙促皮肤损伤修复多肽基因核苷酸序列的成熟序列为第217-264位核苷酸，其氨基酸序列为：

Hylareleasin3：

Gly Leu Leu Asp Pro Val Thr His Ile Leu Gly Gly

Phe Leu Arg Arg

1                   5                       10

15

Hylareleasin4：

Gly Leu Leu Asp Pro Val Thr Asn Ile Leu Gly Gly

Leu Leu Arg Arg

1                   5                       10 

15

4.权利要求1所述的华南雨蛙促皮肤损伤修复多肽，作为制备治疗促进血管内皮细胞和表皮细胞增殖及组织再生，对体表创伤、烧伤和溃疡制剂的应用。