[发明专利]具有α淀粉酶活性的酶及其使用方法有效
申请号: | 02808640.6 | 申请日: | 2002-02-21 |
公开(公告)号: | CN1513059A | 公开(公告)日: | 2004-07-14 |
发明(设计)人: | W·卡伦;T·理查森;G·弗雷;K·A·格雷;J·M·肖特;J·S·克若娃夫;E·J·马瑟;M·斯卢斯卡 | 申请(专利权)人: | 戴弗萨公司 |
主分类号: | C12P21/06 | 分类号: | C12P21/06;C12N9/00;C12N9/24;C12N9/28;C12N1/12;C12N15/00;C08B30/04;C07H21/04 |
代理公司: | 北京纪凯知识产权代理有限公司 | 代理人: | 赵蓉民 |
地址: | 美国加利*** | 国省代码: | 美国;US |
权利要求书: | 查看更多 | 说明书: | 查看更多 |
摘要: | 本发明涉及α淀粉酶和编码α淀粉酶的多核苷酸。并且,也提供了设计新的α淀粉酶的方法和这些α淀粉酶的使用方法。这些α淀粉酶在酸性和碱性pH值和升高温度下具有增加的活性和稳定性。 | ||
搜索关键词: | 具有 淀粉酶 活性 及其 使用方法 | ||
【主权项】:
1.一种分离的核酸,其包括编码具有α淀粉酶活性的多肽的序列,其中所述系列选自:SEQ ID NO.:1,3,5,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,89,91,93,95,97,99,101,103,105,107,109,111,113,115,117,119,121,123,125,127,129,131,133,135,137,139,141,143,145,147,149,151,153,155,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,211,213,215,217,219,221,223,225,227,229,231,233,235,237,239,241,243,245,247,249,251,253,255,257,259,261,263,265,267,269,271,273,275,277,279,281,283,285,287,289,291,293,295,297,299;与如下序列在至少大约100残基的区域内具有至少50%序列同源性的变体:SEQ ID NO.:1,3,5,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,89,91,93,95,97,99,101,103,105,107,109,111,113,115,117,119,121,123,125,127,129,131,133,135,137,139,141,143,145,147,149,151,153,155,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,211,213,215,217,219,221,223,225,227,229,231,233,235,237,239,241,243,245,247,249,251,253,255,257,259,261,263,265,267,269,271,273,275,277,279,281,283,285,287,289,291,293,295,297,299,正如用序列比较算法或通过目测所确定的;与如下序列互补的序列:SEQ ID NO.:1,3,5,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,89,91,93,95,97,99,101,103,105,107,109,111,113,115,117,119,121,123,125,127,129,131,133,135,137,139,141,143,145,147,149,151,153,155,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,211,213,215,217,219,221,223,225,227,229,231,233,235,237,239,241,243,245,247,249,251,253,255,257,259,261,263,265,267,269,271,273,275,277,279,281,283,285,287,289,291,293,295,297,299;和与如下序列在至少大约100残基的区域内具有至少大约50%序列同源性的变体互补的序列:SEQ ID NO.:1,3,5,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,89,91,93,95,97,99,101,103,105,107,109,111,113,115,117,119,121,123,125,127,129,131,133,135,137,139,141,143,145,147,149,151,153,155,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,211,213,215,217,219,221,223,225,227,229,231,233,235,237,239,241,243,245,247,249,251,253,255,257,259,261,263,265,267,269,271,273,275,277,279,281,283,285,287,289,291,293,295,297,299,正如用序列比较算法或通过目测所确定的。
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