[发明专利]美丽鸡血藤EST-SSR标记无效
申请号: | 201210318966.0 | 申请日: | 2012-09-03 |
公开(公告)号: | CN102807986A | 公开(公告)日: | 2012-12-05 |
发明(设计)人: | 李丽;李志英;徐立;黄碧兰;李克烈 | 申请(专利权)人: | 中国热带农业科学院热带作物品种资源研究所 |
主分类号: | C12N15/11 | 分类号: | C12N15/11;C12Q1/68 |
代理公司: | 海口翔翔专利事务有限公司 46001 | 代理人: | 刘清莲 |
地址: | 57173*** | 国省代码: | 海南;66 |
权利要求书: | 查看更多 | 说明书: | 查看更多 |
摘要: | |||
搜索关键词: | 美丽 鸡血藤 est ssr 标记 | ||
1.美丽鸡血藤EST-SSR标记,其特征在于:所述的微卫星标记编号为:Ndl_001、Ndl_002、Ndl_003、Ndl_004、Ndl_010、Ndl_011、Ndl_013、Ndl_015、Ndl_019、Ndl_020、Ndl_021、Ndl_022、Ndl_028、Ndl_031、Ndl_032、 Ndl_033、Ndl_038、Ndl_042、Ndl_043、Ndl_047、Ndl_049、Ndl_050、Ndl_051、Ndl_053;
其核苷酸序列分别为:
Ndl_001:
ctcactcgta gccgttccgc tatcctcagc ataagcatct cgacacattc gatctctaga 60
atcttcttat tatccttctt tatctttcta ctatggcttg ctccaccgtt gtgttgatgc 120
tagtcgctgc attgttggtg agctccaccg ttgctcaatc tctggcatcg tcgccggctt 180
cgtctcccac caactcgcct gtggccactc cgccgaagtc ttctcactct ccggcgccgg 240
tgatttctcc gtccgctagt tcaccggcgg catctcctcc tgctccggtg gagagcggtc 300
cctctccttc gcctgccgcc gtcaactctc cgccatctcc tcctccggct gcctctgcgt 360
cgccagctgg cactccttcg gtcactccct cttcgatctc cagtccgcca tctgaagcac 420
catcaccaag ccaaaacgac gccgctttga acagattcac cgtcgccgga tctgctgctg 480
ttgtggtttt cgctgcggct ttgctgatgt agagagagag agagagagag agagagacag 540
agttacttgg agtcaataat tttcttcctt ttttttaatt ctatattcac gaaattatat 600
ggatcgtgaa cccttgtacc tcatttttgg ttgctcttct cagtgtgacg attcattctt 660
tcgattattt tctgtattct atttattatt ctatga 696
Ndl_002:
ggaagactcg gagtgattga tggaggcaat gctttcaatc aatttgttgg tgtagagttc 60
gatagttatg tcaatgggtg ggatccgaat tctgcacata ttggaatcga cgtcaactct 120
ttgatttcgt tgaagacagt gaaatggaac agggtgagtg ggtcattggt ccaagtgagc 180
ataatctatg actctctgtc taagacattg agtgttgctg tgactgacaa gaatggtcaa 240
atttctaccg ttgctcaagt cgttgatttg aaagctgtgc tcccggagaa ggtcaaggtt 300
ggtctatctg ctgccacatc ttcgggcggc cgtcaaagac acgacatcca ttcatggtct 360
ttcacttcaa acttggagac ggctacactc agcaccgcgg agaacgcaaa tattgcaagc 420
tatgcatgat tcctggtccg tcaaatgtgg cttgtgataa ctagtatttc taaataaagg 480
ccatatatct agtcaatata tatatatata tatatgtgtg tgtgtgtgtt tgagtccatg 540
catgttgctt aaattttggc tactcaactt atgcaaggtg ggtggttgaa aatggaatta 600
tgtttgtcct actcgaa 617
Ndl_003:
gcacccccca ctcccttccc ctctctctcc tattatcgct tccgtacaac aaatcatgtg 60
agattactgc ttaccgtctc taatctctac cctctccttg cccagtattt ggtggtgtaa 120
tcatcgctag ccagctcttc cttcacaatc ctccaaaaaa cacccttccc acttcgacat 180
ctcttagcta gatttcaaca taaatgaaat tttacttatg cagctgaagg tgtcttccat 240
accataatat atatatatat atccattata tatcaaaata ttagttttta gggttttaag 300
ttgtgcacct ttctacgaat ttgaaataat taaaggatta tgctagtagt acctgttgat 360
tgaagaagct cttgttcagc tgggttagat atagttgagt gagagagttt tgatttctaa 420
gtggaaaaat taaatggagg aatacaccag taatcacatg agcgaaaaca caagtccaag 480
tccaagggca aatttgttct actccccggc cggtgattca agcgtaggaa accggcatca 540
tgactttccc atcaacaact ttcatcttca attgggtgga tcggatcact gctttcaatc 600
tgatcaagta ccacaccctt ccgtgaaaac cgagtccaac aactcgcagc tccaccctcc 660
tattttccac tatccgctga tgatgagagg aaatct 696
Ndl_004:
gaatccggca cgaggctttc ccacactctg cagcaacgta actgcttctc ccaaattacc 60
aaaaaaacaa aagagaaata aatatttagt aagaattagt tacgtgacgt gttattatta 120
tggcgccgag ggataagacg ggtggcgtta aggtgaacgg caacggtaac ggcggcgtga 180
aggaggtgca ttttcgggga gtgaggaaga ggccgtgggg gagatacgcc gcggagatca 240
gagatcccgg caagaagagc cgcgtttggc tcggcacctt tgacacggcg gaagaggcgg 300
cgcgagccta cgacgccgcc gcacgcgagt tccgcggccc caaggccaaa accaacttcc 360
ccttcccttc cgacaatgtc aagatccaga gccccagcca gagcagcacc gtcgaatcct 420
ccagccgcga ccgcgacgcc gccgccgccg ccgccgattc ctccccgctc gacctcaact 480
tggcccccgc cgccgtcgcc gcccccgttc ggttcccctt ccagcatcag ttcccgccga 540
tgccgcccgc aaaccaggcg ttttacttcg acgccgtcct acgctccggc atgctgggtt 600
cccttccgtg ccagggcttc gggttcaatt ataaccaccc cgtggcggcg agtgagtttc 660
acgcgacggc gggagcgcaa agcgactcg 689
Ndl_010:
cggcgacgcc gttactgcgg ggacccaagg agatgaaagc ggggcagcgg acgtagaggt 60
ggtaacggcc ggagatgaag gcgccaacct tccaacggac cctgccgtca atcttgacgg 120
tgacgaggac gttaccgttg gactggtcct gagtgaggcc cacgaagttg aagggggcga 180
cggggacgtc ggtgccgtag acaaacggcg accaaacgtc gacctctttg tggccttggt 240
aagagggagg gatggcggtg cggtaagtga cctgctggct ccggtaactg acgtaggtgt 300
cgagacggtc gtagtagact ccgatgtggt cgttggggtt gcgcgaggag agggtgactt 360
ggaagttgga ggttaagaag tttggtacgg tggcgttgaa ggcgtaaacg gtgacgtctt 420
ggaggatgaa ggatggcttg gagggtctta ggattgccca tatgagaagg attgctacga 480
ggacgatgaa gaggaatact atgattcccc agaatatgcg ccggaagatc ctcctcttcc 540
tccccttgtg gtgctcgcac tccttcaccg acatgattat gcgctcttct tcttcttctt 600
tattttttaa ggtgaagtga aagtgaagag tgggggcgag tgtgtttagc tgaaactatc 660
tatgaagaat aattaagata ttgattggga caagagcggg ggactagaag tctagaagtg 720
aggagcaacg gcagaggaaa ttatagaatg attttgtttg tgaggggaag gaattatggg 780
gaggtttcg 789
Ndl_011:
caatctagat tctaaaacca gcgaatgagg cccaagtcca actactatgt acaggaacaa 60
attaatccca actaactgaa aaaagaggga aaaaaaaaaa actacggagt aattaatttg 120
gatctgttga ttagtattgg gggattttat ttttggtgac tagtagtggg ggatttcaag 180
cttgggtatg gtgaaaagac ggggcttctt cgctggatga gggctggaga cctcgtcgtc 240
cacgaagaac ttgttggaaa agtccggaaa agccgggagg ttgagatcga agtcgcggtg 300
gtggctgtga gaggaaccaa ctccttccga ggcagtgacg gcgctgttgc cgccaccgcc 360
gccttcgtag tggcaacgct tgtgtcctcc caaggcctga cctgtgggga aggacttgtg 420
gcagatggaa cactcatggg tcctaactcc accggtggat gcggaactgg tcgtcacggc 480
ggaggaagtg gactgatcct cggcagtggc cccgccggcg agtttccggt ggctggcctt 540
gtgtccgccc agcgcttggt aagaggagaa ggctttgttg catacggagc acttgtaact 600
gagcttggcg gtggtggtgg tggtggttag cggcgccgac tcattatcag atagagcagg 660
cttgacggta gggacagtgt caccgccgcc gccgccgccg cgagcgagca tgatgaggca 720
gagagcgagg tactcttcct cggtgcatga agaggggtgg tcgatgcggc cacgcttgga 780
acgcttgcgc ttcgcccatg gtgtatccag gtagctgaga ttggcttcct caaacggact 840
gaaggatggg gtggcggtgg tgggtgattt cagtgcttcc agagccatct gatcgatcgg 900
ggctactcaa caacgaagag agtgcgaaag agaagagagg tggtgcttct gctacttggg 960
tttgtgtttt 970
Ndl_013:
ctggtccgag cctcaacact gaacgcgcaa aggaaaaagg ggattagggt ttacggaagc 60
gctaatggat ttgatagcgg agaagaagga atcagagaac aacaacaaca acaacagcaa 120
caaccccgaa aatgcccacc aattcgtaga ttcttcgtct caattggcaa ctgatgccaa 180
caacaacaaa gaagccgaag agcgacaagc acgtgaactc aaagccggtc ttcaccctct 240
aaagcacaaa tttgtatttt ggtacacgcg tcgaacacct ggagttcgaa atcaaacatc 300
atatgaggac aacataaaga aaattgttga tttcagtacg gttgaaggat tttggatttg 360
ctattgccat cttgctcgcc cttcttcttt gcctagtcca acagatttgc acctttttaa 420
ggaaggaatt cgtcctttat gggaggactc tgctaactgc aatggtggta aatggattat 480
acgattcaaa aaggttgtct caggtcgttt ttgggaggac ctggttcttg cattagtggg 540
tgaccaattg gattatgggg ataacatatg tggtgcagta ctaagcattc gtttcaatga 600
ggatatattg agtgtctgga atcgcaatgc atctgatcat caggctgtaa tggctttgag 660
agattcaatt aagcgtcact tgaagcttcc tcacagctat gttatggagt acaagcccca 720
tga 723
Ndl_015:
gagagctatc aattagtctc tttctctttc tcagagggat ggcacgaact gccttggatt 60
tttcttcacc ctttcaacga cctaacccaa cagcaatcat catgccctca tcctccttgc 120
ccttctctct gaccgtccat agccatcatc attaccccct caacgctccc acctcttcct 180
ttatcagaaa catcatcatc atccaccaaa cttttgctga tgatgatcac cttcaccatc 240
acaacaagca ccactcaaaa caagctcaag aagaagcaca agaagaagac gacgaagaca 300
aagaaggcaa agttgttgct gctttgctta cgcttacgag aaaaacccta ctttcatatc 360
tagcttcggc aacttataat attgagtgaa aagggtctag cttttgctac aaagatccaa 420
caactcaact ctgggggaat caagcgtctc ggtccgatcc atttctgttt tggttaagta 480
tattccatgt taccaactat cctttttctt ttgtttttat tttataccaa attttagttt 540
gatttgcaga aggattatgt tagctactta gcttgcttta tcactttctt ccttcctctc 600
tctctctctc tctctctccc cttctctctt tctgggtgta gaagtgttat tactttttgt 660
tgatcaaaaa attaaaagcg ttattactta tcgtaaaaaa aaaaaaaaaa aactcgag 718
Ndl_019:
cccagctgtg tgaactttct tgtgtaacca gtagagacag cggggggcaa ccttaaagag 60
ttaattttcc agagagagag agagagagag agagagagag agatatgttg gggatcttca 120
gcagctccat cgtgtcgccg ccggaagagc tggtcgccgc cggcagccga actccgtcgc 180
cgaagacgac ggcgggggcg ctgctgaaac ggttcgctga gagcaaggcg tccacagtga 240
ccatgcagat cggggagcag gcccagctgg cttacacaca ccacaacgag tcaccgtggc 300
aacccagatc atttgctgtg aaggatgaga tattctgcat gtttgaggga gctcttgaca 360
atttgggcag cttgagacaa cagtatggac tcgccaagtc tgccaatgaa gtacttctgg 420
tgattgaggc ttacaaagct ttacgtgacc gagcacccta ccctcccaat cacgttgttg 480
gccatctcag tgggagcttt gctttcgtag ttttcgacaa 520
Ndl_020:
cgaaacctcc ccataattcc ttcccctcac aaacaaaatc attctataat ttcctctgcc 60
gttgctcctc acttctagac ttctagtccc ccgctcttgt cccaatcaat atcttaatta 120
ttcttcatag atagtttcag ctaaacacac tcgcccccac tcttcacttt cacttcacct 180
taaaaaataa agaagaagaa gaagagcgca taatcatgtc ggtgaaggag tgcgagcacc 240
acaaggggag gaagaggagg atcttccggc gcatattctg gggaatcata gtattcctct 300
tcatcgtcct cgtagcaatc cttctcatat gggcaatcct aagaccctcc aagccatcct 360
tcatcctcca agacgtcacc gtttacgcct tcaacgccac cgtaccaaac ttcttaacct 420
ccaacttcca agtcaccctc tcctcgcgca accccaacga ccacatcgga gtctactacg 480
accgtctcga cacctacgtc agttaccgga gccagcaggt cacttaccgc accgccatcc 540
ctccctctt 549
Ndl_021:
aaaacacaaa cccaagtagc agaagcacca cctctcttct ctttcgcact ctcttcgttg 60
ttgagtagcc ccgatcgatc agatggctct ggaagcactg aaatcaccca ccaccgccac 120
cccatccttc agtccgtttg aggaagccaa tctcagctac ctggatacac catgggcgaa 180
gcgcaagcgt tccaagcgtg gccgcatcga ccacccctct tcatgcaccg aggaagagta 240
cctcgctctc tgcctcatca tgctcgctcg cggcggcggc ggcggcggtg acactgt 297
Ndl_022:
cggcggtgac actgtcccta ccgtcaagcc tgctctatct gataatgagt cggcgccgct 60
aaccaccacc accaccaccg ccaagctcag ttacaagtgc tccgtatgca acaaagcctt 120
ctcctcttac caagcgctgg gcggacacaa ggccagccac cggaaactcg ccggcggggc 180
cactgccgag gatcagtcca cttcctccgc cgtgacgacc agttccgcat ccaccggtgg 240
agttaggacc catgagtgtt ccatctgcca caagtccttc cccacaggtc aggccttggg 300
aggacacaag cgttgccact acgaaggcgg cggtggcggc aacagcgccg tcactgcctc 360
ggaaggagtt ggttcctctc acagccacca ccgcgacttc gatctcaacc tcccggcttt 420
tccggacttt tccaacaagt tcttcgtgga cgacgaggtc tccagccctc atccagcgaa 480
gaagccccgt cttttcacca tacccaagct tgaaatcccc cactactagt caccaaaaat 540
aaaatcgccc aatactaatc aacagatcca aattaattac tccgta 586
Ndl_028
gagccaacag ggccatcaaa ataccagtat ttctactgga tcctcctctt cccaaaagca 60
tgcacagaat cagcaacaaa agcctaataa tgctagtggc tccaatggtg gtggtggtag 120
tggaagcttg caaggttttc ctgtcaacaa aaacccgcca tcacagccac tgcagttgca 180
gcagcagcag cagcagaggc agcagttaca gaatcatcac acttcaaatc cagctcgcca 240
tgttgagtct gagatgggcg gtgaagattg tccgtctact gctgatagtc gtctcgctcg 300
tgctgccatg aatatttatg gccagaattt tgcaatgcca atgcaaacac caaactttgc 360
tttgatgacc cctgcaatta gtggtgctgg ctctaatggc agtcatagtg aaaagaaaca 420
atcacaacag caccctggtc caaaggctgg a 451
Ndl_031:
tttcaacaga agtcaagtgg cggcgttgat ttgccttccc ctgattcagt ttcaagtgat 60
agtagtcttt caaatgcaat gtctcgtcca aagcctgtaa tctatcaaga ccaagttcag 120
attcaatccg gagctacaag ggttcctagc aaccccgtgg atccgaaact caatgtgtct 180
gattcacacg gtcggatcca ggtgcagcag caacatgtac aggacccggg atatttattg 240
caacagcaat tcgatcacca ccagcaacag caattcgatc accaccagca gcagcaattt 300
gatcaccgcc accagcagca gcagcagcag cagcagcagc agtatgtaca tggcacacac 360
ttcattcacc ataaccctgc aattcctgca tactatcctg tatatccttc ccagcaacag 420
ccccaacccc aggtttacta tgtgtctgca agacaggccc aagcttacaa cttgtctatg 480
cagcaggcta acatgggtga atctgc 506
Ndl_032:
ccttgatgct gttaatttgc ataagggtaa gcttcttttt atcatttttc atcatttcat 60
tctcaatatg atgatatgat atgatccttt tttttttttc tcaattcaat caagtaaatc 120
acttttacaa caacaccaac aagtttttgt caattctctt ttgttaaatg ttaattcttt 180
ggtgttatat ttatttattt atttatttat ttttatcaat ttcattttat tgtgcatgct 240
attgcaatcc gttttgatca cttccaagtg attgattcgc gatctccttg gataaaataa 300
ggataaccat ggatgacact tttttgtttg ttactttttt ttataaataa aatatagagt 360
aaaaggggta tagtgataga ggaggcgaaa atgcggcttt gacttggacc aattgtaatg 420
ggatgggttg agagggatac tctgaaaatg tgccatgtgt gagtgtgagt gatgttgtca 480
catatctaac tgtagctcag ttgttca 507
NdL_033:
cagatgatca atgttcagaa caagaactcc tccttggtgc tcatcttgcc tctgaacatg 60
gctgaagcag tggctcacca ggccctaacc tctggagcac tcaaaaccca aaaccctaga 120
aagagaaaac aaaaatccaa acaaaaatct ctccctcaga gagagagaga gagagagaga 180
ggaggccatg gatttgcaac agagcgagtt cgatcgcctc ttgttcttcg agcatgcacg 240
taagactgca gaagctgaat acgccaagaa ccctctcgat gctgacaact tgacaagatg 300
gggtggagct ttgttagagc tgtctcagtt tcagagcttt cccgagtcaa agaagatgac 360
ccaagatgct atttcaaagc tggaggaggc gctttctgtg aatcccaaga agcatgatac 420
tctttggtgc ctgggaaatg ctcacacttc acaagcattt ttaatcccag accaggagga 480
ggctaaagtt tattttgata aggcagctga gtactttcag caagctgttg atgaggatcc 540
ttcgaatgaa ctttaccgca aatcattgga agtggctgct aaggctcctg agctgcatgt 600
agaaatccat aagcatggat ttggtcagca gcagcagtca acagggacag ctggaccttc 660
tacttcttct ggtacaagac tca 683
Ndl_038:
ctccctcaaa ccccccaaga ccagcagcag caccagcaac aacaaccgca aggcatcacc 60
atgaacccta atgtctccac cttccaactc caacaaaccc tccaacgctc cccttccatg 120
tctcgcctca accaagttca acctcagcag caacagcagc agcagcagca gttcggcgtc 180
gcgaggcagc agcccggact ttacggcgca cagatgaatt tcggagcctc cgctcagcag 240
cagcaattag gtggcggcgg cgctgccgct tccaacctgt cgcgctcggc gttgatcgga 300
cagagcggcc acttccccat gttgtctggc gccggagcgg cacagtttaa cctgttatcc 360
tcgccgaggc agaaaggtgg gctggtccag ccctcgcaat tctcttcagc taattctgct 420
ggacagccac tgcaaggaat gcaagccttg gggatgatgg ggatgccgaa tctcaactcg 480
caactcaggg ccaacggagc ccttgcttat gctcagc 517
Ndl_042:
cttactcctc tttagattcc attcccaatg gtacgctcat cgctctctct ctctctctct 60
ctgctttcac tcattcgttt ttctctgtta atctctcttt acatacttct ctttatgtgc 120
tttcgctcat tcgttttccg aaatgcacgg gataataaca acaggcttcg gagaagaaac 180
tctcgaaccc catgagggaa atcaaggttc agaaactcgt cctcaacatc tccgttggtg 240
agagcggcga tcgtctcact agagctgcca aggtgctgga gcagctcagt ggccaaaccc 300
cagtattttc caaagcgagg tacactgtcc gttcctttgg tatcagaaga aatgaaaaga 360
ttgcatgcta tgttactgtt agaggcgaca aggcaatgca gcttttggag agtggtttaa 420
aggtgaagga atatgaactt ctgcgaagaa acttcagtga tactgggtgc tttggctttg 480
gtattcagga gcatattgat ttgggaatca agtatgatcc ttcaactggt atttatggaa 540
tg 542
Ndl_043:
aaaagcttct caaatttctc ttttcaaacc cgaccaggac ctcctgctac atccaccaca 60
acaacaacgt ttcaatcttc aaacgttaca gttcaaacgc aacagccatg gagttttcag 120
gaaggcacaa aacaggatag ttattcttct ggaaagagta tgatgaaaac tgaaaactct 180
tcttcgatgc agagtttttc ccctgagatt gctagtgtcc aaacgaacaa caacaacaac 240
aacaacgggt ttcaatcaga ttatagcaat taccaaccac aacaaactca gacgttaagc 300
agaaggtcag atgatggtta caattggagg aaatatggac aaaagcaagt gaaaggaagt 360
gaaaatccta gaagttacta caaatgcaca tatcccaatt gccctacaaa gaagaaggtg 420
gagaggtctt tagatggaca aatcactgag atagtttaca agggtactca taaccatccc 480
aagcctcaat ctactaggag aaactcatca tcttcctctt ctcttgcaat ccctccttca 540
aaccccatca gcacagaaat cccagatcaa tcatatgcca cccacggcag tggacaaatg 600
gattcggttg ccaccccgga gaactcatca atatcaatgg ggggatgatg attttgagca 660
Ndl_047:
ctcaaaatca ttcaaaaccc cgtataagca tagccattga accaagcctg tgccttttct 60
ctctgataat tttccgttgg gtagaagaaa gtctcaacct tttgttcacg aaaagacaag 120
ggatctgaca aggtctagaa ggagagagag agagagagag agaaagaaag atgtgtggag 180
gtgctattat ctccgacttc atcccggcgg cggtggcggc ggggggttcc cggcgcgtga 240
ccgccgacat cctgtggccg aatttgagga aacccagttc gaggaagcag ttgtcgaagc 300
cactgttgtt tttggacgat gatttcgagg ctggtttcag agaattcaag gacgattctg 360
acttcgatga ggacgaggag gaggaagatg atgacttggt tgtcggtgtc aagccctttg 420
ccttcgcttc caccaagacc aaaccttcca agtccctctc tcggggatca acggctgcaa 480
aatctgtgga atcaaatgag caagctgaga cttatggtaa gagaaagagg aagaatcagt 540
atagggggat ccgccagcgt ccatggg 567
Ndl_049:
cacagacaca agcacaaatt ctctctctca catccccaaa ttccttacca ctgactccac 60
accacaccat aacacgctct ccctctctct gctctctact cttttctttc ccgtttccgt 120
tctgggcatt cgcgttggtc acggcgtcat gagctgagga agaagaagaa gaagaagaag 180
aaggaaggaa ggaaggaaga ggaggagcat gggggcgtcg ctatcgaacc tgggtaccaa 240
cggttcgacc atcggtcccg gcctcgggga catacctgag ggctgcgtgg cccgcgtctt 300
tctccacctc accccgcccg agatttgcaa cctcgctcgc ctcaatcgcg cttttcgcgg 360
cgccgcttcc gctgattccg tttgggaagc taagttgccc cctaactacc aagatctcct 420
ccatctgctt ccccatcgcc accagaatct atccaagaag gacattttcg cccttctctc 480
tcgtccctta ccctttgacg acggccacaa gaaagtgtgg ctggacagag tcacgggaag 540
ggtttgcgtg tccatttcgg ccagagcgat ggcaatttac ggggattgat gaccggagat 600
actggaattg gattcccact ga 622
Ndl_050:
agagataatt gtggtgggtt gaaacccggg gttgacgcgt tgtagacatc acggctattc 60
tcagagtttc tcggccgctc tttcatgtcc gaactgtacg acgtaagggt tgtgtagaga 120
gcaacccttc gccgcacggt gggcgtggtg ctttgccttc cgaaggtggt agcccctctg 180
acctcctctt ccttgctggt ggtggtgttc ctgcttctcc tctttccctc cctttctctt 240
tttcctagtt ttctagtttc tagtttaatt taaacttctg gtagttgtta agactgttaa 300
ggtaaacctg ctaaaagaga gactttgtaa tttcccgttg agggtacttg ttgttcttgc 360
tgttgttgtt gttgttgttg ttgttgttgt ggttgggttt tggtgtttag tttgctgaag 420
aaagtgaaaa tgatgttgag aattaagagg gttcccaccg tggtttccaa ttatcaaaaa 480
gaggagtttg gaggggctgc tcgctctgtc gggggctgtg gtcgaaattg cctcaaaagt 540
tgctgtattc aagatgcaaa gcttcctttg tatgcttt 578
Ndl_051:
ctcaaatcat tgcaaacaaa aacaacctta aaaaataaaa ggaaatggaa atggaaagca 60
atctaccagt gataacgaag agagtgtgga gcatggtacg tgtggtattc ttcatgttga 120
gaaaaggcat atcaaagggg aaactaatga tggacctcaa catgatgctc aaacgccgcg 180
gcaagctcgc cggaaaagcc attgccaacc tcatgttcca ccaccaccac caccacggtg 240
gctcctccac ctcaagccgc cgctcccacg actcacaccc gcagttctcc gccccaaggg 300
agtacgagtt cagctgcagc aacactccca actacttctt ctccgcaggg aagcgccacc 360
gcaacaacct cttcacctgc gctcacgcgc cacccacaaa ggacgacgag gcggtgccgg 420
cgaacgcagt gaaggcggtg ctggagatgc taaacaatga agggatggta gaagcatccc 480
ctgcgctgcc agggtttggg cgcagcccaa taatggtgag gcagttgagg gtcacagact 540
cgcccttccc tccgcaagat gatgacaagg acaaccaagt cgataaggct gcggaagact 600
tcataaaga 609
Ndl_053:
ctctctagct cactacacct tttcacgtgt ctctcccttt cttagttctg ttctcttctg 60
tccttccttc cttcactctg tttgtttcct tgtaaaagaa aaaaacctca attgagttta 120
ttattattat ttagtttttc gtttctcatt ttaaggttgt tcttaggcgg tggttgatgt 180
ttctttgtgg ggctgtcttc tcacgtaaaa gtcttacttt gttggaaggg ttgaggtaga 240
gattcttgac gaagaggtta aagggtggta ggcggaaaat ggaaggaaag aaacaaatgg 300
cttcttcttc ttcttcttct tcttcttctt tcacttctga gctctttggg tccaacgagt 360
cacactcaca cccatcctct gcttcttctg gaatttttga atccatgttt tctccatcgt 420
ctaaggtgtt tgggagagag tctctgcgct ctgcagtgac tgggaaaacg accagtgagg 480
gatcgaactc caaaattggt actccaggtt acatcagcaa gggcagtgat ggtgaagctc 540
agagcacagc aaataaggat atgagttcta tttatcagga 580
2.根据权利要求1所述的美丽鸡血藤EST-SSR标记,其特征在于:所述的Ndl_001、Ndl_002、Ndl_003、Ndl_004、Ndl_010、Ndl_011、Ndl_013、Ndl_015、Ndl_019、Ndl_020、Ndl_021、Ndl_022、Ndl_028、Ndl_031、Ndl_032、 Ndl_033、Ndl_038、Ndl_042、Ndl_043、Ndl_047、Ndl_049、Ndl_050、Ndl_051、Ndl_053 24个位点的引物序列分别为:
Ndl_001F:TCTGAAGCACCATCACCAAG
Ndl_001R:TGAGGTACAAGGGTTCACGA
Ndl_002 F:TATCTGCTGCCACATCTTCG
Ndl_002R:AACCACCCACCTTGCATAAG
Ndl_003 F:ACCCTCTCCTTGCCCAGTAT
Ndl_003R:CCCAGCTGAACAAGAGCTTC
Ndl_004 F:TCCGACAATGTCAAGATCCA
Ndl_004R:CCACGGGGTGGTTATAATTG
Ndl_010 F:TCTTGGAGGATGAAGGATGG
Ndl_010R:GACTTCTAGTCCCCCGCTCT
Ndl_011 F:TTGTGGCAGATGGAACACTC
Ndl_011R:TGACACTGTCCCTACCGTCA
Ndl_013 F:AAGGGGATTAGGGTTTACGG
Ndl_013R:GCTCTTCGGCTTCTTTGTTG
Ndl_015F:AAGATCCAACAACTCAACTCTGG
Ndl_015R:TCTACACCCAGAAAGAGAGAAGG
Ndl_019 F:CTGTGTGAACTTTCTTGTGTAACC
Ndl_019R:GGTGACTCGTTGTGGTGTGT
Ndl_020 F:GACTTCTAGTCCCCCGCTCT
Ndl_020R:TCTTGGAGGATGAAGGATGG
Ndl_021 F:GATCAGATGGCTCTGGAAGC
Ndl_021R:GCTTGACGGTAGGGACAGTG
Ndl_022F:CACTGTCCCTACCGTCAAGC
Ndl_022R:TTGTGGCAGATGGAACACTC
Ndl_028F:CTAGTGGCTCCAATGGTGGT
Ndl_028R:AATTGCAGGGGTCATCAAAG
Ndl_031F:TTCAATCCGGAGCTACAAGG
Ndl_031R:TGCAGGGTTATGGTGAATGA
Ndl_032F:GCTGTTAATTTGCATAAGGGTAAGC
Ndl_032R:CAAGGAGATCGCGAATCAAT
Ndl_033F:GGAGCACTCAAAACCCAAAA
Ndl_033R:TACGTGCATGCTCGAAGAAC
Ndl_038F:GTCTCCACCTTCCAACTCCA
Ndl_038R:CACCTAATTGCTGCTGCTGA
Ndl_042F:ATTCCATTCCCAATGGTACG
Ndl_042R:TCTTCTCCGAAGCCTGTTGT
Ndl_043F:GGAGTTTTCAGGAAGGCACA
Ndl_043R:CCTTTCACTTGCTTTTGTCCA
Ndl_047F:GCCTGTGCCTTTTCTCTCTG
Ndl_047R: CTCGAACTGGGTTTCCTCAA
Ndl_049F:ACTGACTCCACACCACACCA
Ndl_049R:TGGTACCCAGGTTCGATAGC
Ndl_050F:GTGGTGGTGTTCCTGCTTCT
Ndl_050R:ACGGTGGGAACCCTCTTAAT
Ndl_051F:TGGACCTCAACATGATGCTC
Ndl_051R:TTCCCTGCGGAGAAGAAGTA
Ndl_053F:CTTAGGCGGTGGTTGATGTT
Ndl_053R:CCAGAAGAAGCAGAGGATGG。
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