[发明专利]一种用于肛肠疾病患病风险评估基因芯片、制备方法及其试剂盒

权利要求书

1.一种用于肛肠疾病患病风险评估基因芯片，其特征在于：包括固相载体、固定在固相载体上的用于检测肛肠疾病易感基因多态性位点的特异性寡核苷酸探针，所述检测肛肠疾病易感基因多态性位点如下：

结直肠癌基因多态性位点(SNP)4个：rs4939827、rs6983267、rs3802842、rs961253

先天性巨结肠症基因多态性位点(SNP)5个：rs137852525、rs104894388、rs1801710、rs193196427、rs193123285

克罗恩氏病基因多态性位点(SNP)11个：rs2066844、rs10761659、rs2066845、rs2066847、rs11209026、rs2241880、rs7714584、rs3197999、rs11190140、rs17234657、rs1893217

乳糖不耐受症基因多态性位点(SNP)3个：rs193268403、rs193267205、rs193116455

溃疡性结肠炎基因多态性位点(SNP)4个：rs2395185、rs9858542、rs10883365、rs11209026、

酒精脸红反应基因多态性位点(SNP)3个：rs193229433、rs193200096、rs193056481

家族性结肠腺瘤性息肉病基因多态性位点(SNP)2个：rs121912665、rs113993961

乳糜腹泻基因多态性位点(SNP)3个：rs2187668、rs6822844、rs6441961

囊性纤维化基因多态性位点(SNP)4个：rs193230349、rs193273640、rs193221728、rs193219992；

所述寡核苷酸探针的核苷酸序列如SEQ ID No.1-78所示，具体列表如下：

表1用于肛肠疾病患病风险评估基因芯片的寡核苷酸探针序列

位点名称       探针序列(5’→3’)

rs4939827      1W-AAAAGAGGAAACAGGACCCCAGAG

               1M-CCAAAAGAGGAAATAGGACCCCAG

rs6983267      2W-AGCAGATGAAAGGCACTGAGAAAA

               2M-AGCAGATGAAAGTCACTGAGAAAA

rs3802842      3W-AAAATCTATCCCAGAAATTCA

               3M-AGACCCATAGAAAATCTCTCCCA

rs961253       4W-ACATCAAGTAAATTCAGA

               4M-ACATCAAGTACATTCAGA

rs137852525    5W-GTTCAGTACCGCATGCAGTG

               5M-GTTCAGTACCGCGTGCAGTG

rs104894388    6W-TTCATACAGAAAGCCTCCGTG

               6M-TTCATACAGAAAGGCTCCGTG

rs1801710      7W-AGACCTTATGGCCCAAGAGTT

               7M-CCAAGGGTTCCAACGCCAG 

rs193196427    8W-TTCTCATAGCCTACGGACTCGAAC

               8M-ATAGCCTATGGACTCGAACCGG

rs193123285    9W-ACCCTGTCTCGTGGACCGC

               9M-CCCTGTCTCGTGGACTGCCCGG

rs2066844      10W-CCGGAGCAGGGCCTTCTCAGAT

               10M-CCAGAGCAGGGCCTTCTCAG

rs2066845      11W-TGGTGCAACAGAGTGGGTGACGAG

               11M-TGGCGCAACAGAGTGGGTGACGAG

rs10761659     12W-AAACTATAACAGAAGGAAAACAGG

               12M-AAACTGTAACAGAAGGAAAACAGG

rs2066847      13W-CTCCTGCAGGCCCTTGAAA

               13M-CTCCTGCAGGCCCCTTGAAAG

rs11209026     14W-CAGATCATTCCAAACTGG

               14M-CAGATCATTCCGAACTGG

rs2241880      15W-ATGAGCATCCACATTGTCC

               15M-GATGAGTATCCACATTGTCC

rs7714584      16W-ACCTTTTAAATTTATTGCTCTGTC

               16M-ACCTTTTAAATTTGTTGCTCTGTC

rs3197999      17W-CGCAAGGTCCCGCTGGCCA

               17M-GAGTATGCGCAAGGTCCTG

rs11190140     18W-TTCAATAGGCGGAAAAGAAGGCT

               18M-TTCAATAGGTGGAAAAGAAGGC

rs17234657     19W-CCAGTCACGTTGTCAAATAGCTTC

               19M-TCCTCCCAGTCACGTTTTCAAATA

rs1893217      20W-TCTTCTTCCTCTACCTCTGTCCCT

               20M-CTTCTTCCTCTACCTTTGTCCCTA

rs193268403    21W-GAGCCACCATGCCCGGCCGG

               21M-GAGCCACCATGCCTGGCCGG

rs193267205    22W-CTTCAGTGATGGACGAGGAGGTGG

               22M-CAGTGATGGATGAGGAGGTGGACA

rs193116455    23W-GTGTCTTTGCCCAAGTACTTCTG

               23M-GTGTCTTTGCCCAAGTATTTCTG

rs2395185      24W-GCCAAAAATTTGTCTTCCC

               24M-GCCAAAAATTTTTCTTCCC

rs9858542      25W-CAAACTGACAGAAGGTATGCACT 

               25M-AAGCTGGCAAACTGACGGAAGGTA

rs10883365     26W-CAGACGGTTTGAAGGTATTTGTGC

               26M-CAGACGGTTTGAAGGTGTTTGTGC

rs11209026     27W-AACAGATCATTCCAAACTGGGTAGG

               27M-AACAGATCATTCCGAACTGGGTAGG

rs193229433    28W-CACTCCCCATTCCTCCCACTACCA

               28M-CCCCATTCCTCCCCCTACCAG

rs193200096    29W-AGTGGCTGGCCCTGGTTCTG

               29M-AGTGGCTGGCCTTGGTTCTG

rs193056481    30W-GGTGCCAGCCTAATTTCTATTAA

               30M-GTGCCAGCCTCATTTCTATTAATT

rs121912665    31W-GGTCTGGCCCCTCCTCAGCAT

               31M-GGTCTGGTCCCTCCTCAGCAT

rs113993961    32W-TGCTTTTAATACGGTAGAAATTGG

               32M-GCTTTTAATAGGGTAGAAATTGGC

rs2187668      33W-GGCAGCTGAGAGTAAATGAGGACC

               33M-GAGGCAGCTGAGAGTAAGTGAGGA

rs6822844      34W-TCCATAGCAAAAAGAGAGGACT

               34M-TCTCCATAGCAAAAATAGAGGAC

rs6441961      35W-GGCAGCCTTGATAACATCTCCA

               35M-GTAGCCTTGATAACATCTCCAGCA

rs193230349    36W-GTAACTAACCCATTCTATTTT

               36M-GTAACTACCCCATTCTATTTT

rs193273640    37W-TAGTGTTGGTGGCAGGAAGTAGAA

               37M-TAGTGTTGGTGTCAGGAAGTAGAA

rs193221728    38W-GTGTTCGACTGCATGGGGA

               38M-GTGTTCGACTGCATGGGTA

rs193219992    39W-TTTGATAGAACTATACACGGAG

               39M-TTTGATAGAACTATACATGGAG。