[发明专利]小麦抗病相关基因TaEDR1及其应用

摘要

本发明涉及植物基因工程技术领域，特别是涉及一种小麦抗病相关基因TaEDR1及其应用。本发明的小麦抗病相关基因TaEDR1是从对白粉病抗性程度很高的小麦抗病新品系“99－2439”中分离的，该基因全长cDNA为3050bp，包括70bp的5′－非编码区，70bp的3′－非编码区，2877bp的编码区，终止子，和30bp的多聚腺苷酸尾，3′－非编码区包含1个典型的加尾信号序列，该基因所编码的蛋白是一个全长由959个氨基酸组成的促分裂原蛋白激酶激酶激酶。本发明的小麦抗病相关基因TaEDR1可以应用在植物抗病改良上、合成新的抗病相关基因上以及小麦基因芯片制作上，具有很高的实际应用价值。

主权项

1.一种小麦抗病相关基因TaEDR1，其特征是：TaEDR1基因的全长cDNA序列，其核苷酸序列的碱基组成：896 a 630 c 758 g 766 t1 cgccaaactg aaataaccaa gagaggaaat attttcgaaa cccaaaccct aggtgctcgt61 ggggagcgcc atgaagatcc cgtttgtgac caagtggtcg caccgatcca gcgagcccgc121 ggggccgtcg aattcggctg cagcgcagca gcagcagcag cagcaggcgc cgtctcctcc181 tcctcccgtg gcgtcgacag aggcggcagg ggatgagttc attctgcagg aggaagagta241 ccagatgcaa ctggcgttgg cgctatcagc gtcggcgtcg ggcgccgagg gcgctgggga301 tcccgacggg gagcagatca gaaaggcgaa gctgatgagc ctcgggaagg gccacccggt361 caccaacagc gatcgtggcg ggggagacac cccggagtcg ctctcccgcc gttacaggga421 ctataacttt cttgattaca atgagaaagt aattgatgga ttctacgacg tatttggcct481 ctctgcggga tcatctgggc agggcaaaat accttcactg gcagagcttc agatgagcat541 tggggatctt ggatatgaag taattgtggt tgactataaa tttgataatg ctctgcagga601 gatgaaggaa gtagcagaat gctgcctgtt gggctgtcct gacattacag tattggtgcg661 acgaatagct gaagttgttg cagatcacat gggtggtcca gtgatcgatg caaatgaaat721 gatcactagg tggttgagca aaagcattga gcagaggaca tcacaccaga caagccttct781 gcatattggc agtatagaga taggcttgtc tcgccatcgt gccttacttt tcaagattct841 tgctgatatt gttggtatcc cttgcaagct ggttaaaggg agtcattaca ctggtgttga901 agatgacgct attaacataa taaagatgga tgacaaaagg gagtttttgg tggatgttat961 ggctgctcca gggactctca ttccagcaga tgtctttaat tcaaagggta ctccattcaa1021 cttcagtcaa acattgggtc agaatcaggt ggtggagtca gcaagtaaca tcgaagatga1081 cccagttgca ttacagtcag agcataaacg taaccaaggg catatgtttg ccaataataa1141 tcggatctca gtcaatctat caagctatga gaatacaatg accgctggaa gtagtgctag1201 tgaacctggg acattggacc ctaggatgca attaggtaaa acatcaactt tgcctagtgc1261 tccttccaag cagaagaaga atctgcaatt gattacagac tctcatgaaa ctgaagagtc1321 ccgaaaacta tttgtggagt tagatccttt caatgctatt gaatctggga aaagctcatt1381 ggcattcaag ggattaaata atagaaacaa tgaattccaa aggcgtagag agaatgtagt1441 cccaccatct gtaagatctc aacagccatt ggtgatgaaa aactggtctg cttgcaatga1501 catttccaac aacaagcaat acaatgttgc tgatgggtca gttcctcgga gaaatgccac1561 tgacaatgca tcgtcatctc agttggcgtt gtcaactgca aagcattaca attccaatgt1621 tagagagcta aacgatagag tgtatgcagc acctgctcgt aattatgaca acaagatagt1681 tggtacctcg gctatggcca aagcattgac tggagagtgc cctgacagat cacaggtgcc1741 acctggtctt tattatgaca agatgcttgg tacctcttct atgaatgcag cttctacatc1801 cggaatcggg aaagttgcag aaaaggaccc tcataatgat ccgggaaaag gtcccatcta1861 ttctagattt gatggtgaac tttctaaaaa tgctcaagga tttactcccg aaagggatga1921 gcacaaggaa aattgtggca gtcatgacca caaaatgtta tatcctgatc caagaaagtc1981 ccctcttgac agattcatgg acaggccaag gcagagcata gaatgtgttt ttccatccca2041 agttggatca aataaggctg acatggtgtt ggatgaagtg tctgaatgtg aaatcctttg2101 ggaagatctt gtaatcgatg aaagaattgg cataggttca tatggagaag tctaccatgc2161 tgattggaat ggaactgaag tagctgtaaa gaagttcttg gatcaagagt tctatggtga2221 tgctttagag gaatttcgtt gtgaagtgag gattatgcgt cggctccgtc atccaaatat2281 tgttctcttt atgggtgcag taacacggcc tccacactta tctattgtat cagaatatct2341 tccaagggga agcttatata agatcattca tcgccctaat tgccaaatcg acgagaagcg2401 taggattaaa atggcccttg atgtggccag aggcatgaat tgtcttcata ccagtgtacc2461 aacaattgtt caccgggatc taaaatcacc aaacttgctg gttgacgata attggactgt2521 gaaggtctgt gatttcggac tttcacgtct gaagcacagt acatttttgt catcaaaatc2581 cactgccggg actcctgagt ggatggcacc agaggttttg cggaatgagc aatccaatga2641 gaagtgtgat atttacagct ttggtgttat cctgtgggag ctagcaacac taagaaagcc2701 atggcatggg atgaaccaaa tgcaagttgt gggcgcagtt ggcttccagg accgacggct2761 tgacattcca aaagaagtag atcctatagt tgcatcaatt atacgtgatt gctggcagaa2821 ggatccaaac ttgcgtcctt ctttcatcca attaactagc tacctgaaga cattgcaaag2881 gcttgtaatc ccttcacatc aggagacagc gagcaaccat gtaccctatg aaatatcttt2941 atatcggtga accgcacatc tctaccccgg ggttgtcacc caccaagtgt gaataagcag3001 taattatgat tttgtcatcg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa