[发明专利]一种稳定性好、准确度高的α-羟丁酸脱氢酶检测试剂

权利要求书

1.一种稳定性更好、准确度高的α-羟丁酸脱氢酶检测试剂，其特征在于包括试剂R1和 试剂R2，所述试剂R1和试剂R2的组成如下：

1）试剂R1中含有

缓冲液_{····················································}100mmol/L，

BSA_{·······················································}1g/L

2-酮丁酸_{··················································}4.4mmol/L，

EMULGEN-707_{···············································}0.7ml/L

羟基乙叉二膦酸_{············································}1ml/L-5ml/L

十八烷基三甲基溴化铵_{······································}0.1g/L-1g/L

烷基酚聚氧乙烯醚(APEO)_{···································}2g/L

抗坏血酸氧化酶_{············································}1KU/L-5KU/L，

胆红素氧化酶_{··············································}1KU/L-3KU/L，

蔗糖_{······················································}5g/L

海藻糖_{····················································}5g/L

黄原胶_{····················································}3.8g/L，

防腐剂_{····················································}0.5ml/L-1ml/L；

2）试剂R2的组分为：

缓冲液_{····················································}100mmol/L，

NADH_{······················································}4.4mmol/L，

黄原胶_{····················································}3.8g/L，

防腐剂_{····················································}0.5ml/L-1ml/L。

2.根据权利要求1所述的α-羟丁酸脱氢酶检测试剂，其特征在于试剂R1中缓冲液为25 ℃，pH为7.3的磷酸盐缓冲液。

3.根据权利要求1所述的α-羟丁酸脱氢酶检测试剂，其特征在于试剂R2中缓冲液为25 ℃，pH为3.4的酒石酸缓冲液。

4.根据权利要求1所述的α-羟丁酸脱氢酶检测试剂，其特征在于所述防腐剂为PC-300。

5.根据权利要求1所述的α-羟丁酸脱氢酶检测试剂，其特征在于所述添加的稳定剂为 黄原胶。

6.根据权利要求1所述的α-羟丁酸脱氢酶检测试剂，其特征在于所述金属离子螯合剂 为羟基乙叉二膦酸。