[发明专利]运用比较基因组学快速鉴定草莓白粉病基因

权利要求书

1.草莓抗白粉病基因，其特征在于选自下列3个基因或其之一：

gene09623-vl.0-ab_initio

氨基酸序列：

MAAAGKERSLEQTPTWAVAVVCFILVLVSIIIEYILHLIGKWLERKNKRALYEALEKIKAELMLLGFLSLLLTVGQGPISDICISKSVGATWHPCSKKQEPVYAKEDGESTSSESDDDSGRRRLLSVVLSSSGKNARRVLAAAGYDKCGAKGKVPFVSFYGIHQLHILIFVLAVSHVLYCIITLVLGRAKMRKWKTWELETRTADYQFSHDPERFRFARDTSFGRRHLSFWSRSPILLWIVCFFRQFVRSVPKVDYLTLRNGFIFAHLAPQSQTKFDFQKYINRSVEEDFKVVVGISPVIWLFAVLFLLSNTHGSWSYLYLPFIPLVMILLVGTKLQVIITKMGLKIQERGEVVKGTPLVEPGDHLFWFNSPRLILHIIHFVLFQNAFQLAFFAWTWYEFGLKSCFHDKMEDVVLRITMGVVIQILCSYVTLPLYALVTQMGSTMKPTIFNDRVANAIRKWHHAAKKHVKQSKHSVPTSAPGTPLHGMSPVHLLRHYHSELDMDSMQTLPRMSYAEREGPNESPSHHDTDDVPWSNQGQEEEISAHYQPNSVNNPPPGYGNQIQHEIQIHTKD

核苷酸序列：

ATGGCAGCAGCTGGAAAGGAACGATCTTTGGAGCAAACGCCGACGTGGGCCGTTGCCGTTGTTTGTTTCATATTGGTTTTGGTCTCCATCATCATCGAATATATCCTTCATTTGATCGGAAAGTGGTTGGAAAGGAAGAATAAAACGAGCTCTCTATGAAGCACTGGAAAAGATCAAAGCAGAGCTTATGTTATTGGGGTTCCTCTCGCTGCTGCTAACAGTAGGACAAGGCCCCATTTCGGACATTTGCATATCGAAGTCTGTTGGGGCTACTTGGCATCCATGTAGTAAGAAGCAAGAGCCAGTCTATGCAAAGGAGGACGGAGAGAGCACTAGTTCAGAATCCGATGATGACAGCGGCCGTAGAAGGCTACTATCCGTGGTTCTATCGAGTTCCGGCAAGAATGCCCGACGTGTTTTGGCCGCAGCCGGATATGACAAATGCGGTGCCAAGGGAAAAGTTCCATTTGTGTCATTTTATGGAATTCACCAGCTTCATATACTCATCTTCGTGCTAGCCGTATCCCATGTGCTTTACTGCATAATAACACTGGTTTTGGGCAGAGCTAAGATGAGGAAGTGGAAAACATGGGAATTGGAAACCAGGACAGCAGACTACCAGTTCTCACATGACCCTGAAAGATTCCGATTTGCGAGAGACACATCGTTTGGGCGAAGGCACTTGAGCTTTTGGAGCCGGTCACCCATTTTGTTATGGATTGTTTGCTTCTTCAGACAGTTTGTTAGATCAGTGCCTAAGGTGGATTACCTGACGCTACGCAATGGGTTCATCTTTGCACATTTGGCACCTCAGAGTCAAACTAAATTTGATTTCCAGAAGTACATCAACAGATCAGTCGAAGAGGACTTCAAGGTTGTGGTGGGGATCAGCCCTGTTATCTGGTTATTCGCCGTATTGTTCCTGCTATCTAACACCCATGGCTCATGGTCCTATCTCTATCTACCTTTTATTCCCCTAGTGATGATCTTGTTAGTAGGGACAAAGCTACAGGTTATAATAACCAAGATGGGGCTTAAAATTCAAGAAAGAGGAGAAGTAGTTAAGGGTACCCCGCTTGTTGAGCCTGGTGACCATCTCTTCTGGTTCAACAGCCCTCGTCTCATTCTTCACATCATCCATTTTGTTCTCTTTCAGAATGCCTTTCAACTTGCTTTCTTTGCATGGACTTGGTACGAATTTGGGTTGAAGTCTTGCTTTCACGACAAGATGGAAGATGTGGTCTTAAGAATTACAATGGGGGTCGTCATACAAATACTATGCAGCTATGTAACTCTACCACTTTATGCCTTAGTTACACAGATGGGTTCAACTATGAAGCCTACGATATTTAACGACAGAGTAGCAAATGCTATAAGAAAGTGGCACCACGCAGCAAAGAAGCATGTAAAACAGAGCAAGCATTCGGTTCCAACCAGTGCACCCGGCACTCCATTACATGGCATGTCCCCGGTTCATCTTCTGCGGCACTATCATTCCGAACTGGACATGGATAGTATGCAAACATTGCCAAGGATGTCATACGCTGAAAGAGAAGGTCCAAACGAGTCCCCATCTCACCACGACACCGACGATGTACCCTGGTCTAACCAGGGTCAGGAGGAAGAGATTAGTGCTCACTATCAACCTAACTCGGTGAACAATCCTCCACCTGGTTATGGAAACCAAATTCAACACGAAATCCAAATTCACACAAAGGATTAG

gene02764-vl.0-ab_initio

氨基酸序列：

MAASTSGRSLEQTPTWAVAVVCFVLVLISIIIEHILELIGKWLKNKHKRALFEALEKVKSELMLLGFISLLLTVGQGPISNICISEKVGNTWHPCSKKQEVKIEEEEEVESPSRRLLAAFESGYGGGNVRRVLAAAGTDKCAAKGKVPFISADGIHQLHIFIFVLAIFHVLYCILTMALGRAKVCFFRQFVRSVPKVDYLTLRHGFIMAHLAPQSHQQFNFQKYINRSLEEDFKVVVGISPPIWCFAVIFLLFNTHGWYSYLWLPFIPLIIILLVGTKLQVIITKMGLRIQERGEVVKGTPVVQPGDDLFWFNRPRLILYLINFVLFQNAFQLAFFAWSWYEFSLKSCFHEHIEDIIIRVSMGVLIQILCSYVTLPLYALVTQMGSTMKPTIFNERVATALRKWHHTAKKHIKQNKGSVTPMSSRPGTPSHHASPIHLLRHYRSEVDSFHTSPRRSNFDVEQRYETDSPSPSHHLHIDGSFSHQHQHQVELGNVEHDRMEVNEPNSGHATIRGSQSTREQHEINIGPPKDFSFDKRQAASSSYNMPLVFRLTDPNSMSESEAMVIVEGLLGKLARRCYAVLCLGVPVVSVTVAHFIKGTMDLPEEPTQPRERSKRRLRALQGQLFVPIESSY

核苷酸序列：

ATGGCGGCATCAACCTCTGGAAGATCGCTGGAGCAAACGCCCACATGGGCCGTCGCCGTCGTCTGTTTTGTCTTGGTTCTTATCTCAATCATCATCGAGCACATACTCGAACTTTAGGAAAGTGGCTGAAGAATAAACACAAACGAGCTCTATTTGAAGCACTAGAAAAGGTGAAATCAGAGCTTATGCTACTGGGGTTCATATCGCTGCTGCTGACCGTCGGGCAGGGTCCGATATCGAACATATGCATATCGGAGAAAGTGGGGAACACGTGGCATCCGTGTAGCAAGAAGCAGGAGGTTAAGATCGAGGAAGAAGAAGAGGTTGAAAGCCCGAGTAGGAGACTGCTGGCGGCGTTTGAATCTGGTTATGGAGGTGGAAACGTACGCCGCGTTTTGGCGGCTGCTGGCACCGACAAGTGTGCAGCCAAGGGAAAAGTTCCATTCATATCTGCCGATGGTATTCATCAGCTGCATATATTTATCTTCGTCTTGGCTTTTTCCACGTACTCTATTGCATCCTCACAATGGCTTTAGGAAGAGCCAAGGTTTGTTTTTTCAGACAATTTGTTAGATCAGTTCCCAAAGTTGATTACTTGACCTTGCGGCATGGATTTATCATGGCACATTTGGCACCTCAAAGCCATCAACAATTCAATTTCCAGAAGTACATAAACAGATCACTCGAAGAGGATTTCAAAGTTGTTGTGGGAATCAGCCCTCCAATTTGGTGCTTTGCCGTGATCTTTCTACTCTTCAATACTCATGGCTGGTATTCGTATCTTTGGTTACCCTTTATCCCACTCATTATTATTCTATTGGTGGGAACCAAGCTACAAGTGATCATAACCAAGATGGGTCTTAGAATTCAGGAAAGAGGAGAAGTTGTGAAGGGCACACCAGTTGTTCAACCTGGTGATGACCTCTTCTGGTTCAATCGCCCTCGCCTCATTCTCTACCTCATCAACTTCGTTCTCTTTCAGAATGCCTTCCAACTTGCTTTCTTCGCATGGAGTTGGTATGAATTTAGTTTGAAATCTTGCTTTCATGAGCATATTGAGGATATCATCATCAGAGTTTCAATGGGGGTCCTCATACAAATTCTTTGCAGTTATGTCACTCTCCCCCTTTATGCCCTTGTCACACAGATGGGCTCTACTATGAAACCAACAATATTCAACGAGAGAGTAGCCACAGCTCTACGCAAGTGGCATCACACGGCTAAGAAGCACATCAAACAGAACAAAGGCTCCGTCACCCCAATGTCTAGCCGACCAGGCACTCCATCCCACCATGCATCGCCCATCCACCTCCTGCGGCACTACCGCAGCGAGGTGGATAGCTTCCACACATCACCACGGAGATCAAACTTCGACGTCGAACAACGTTACGAAACCGACTCCCCCTCCCCCTCCCACCACCTCCACATAGATGGTTCATTTTCTCACCAACACCAACACCAAGTCGAATTAGGGAATGTGGAACATGATCGGATGGAGGTCAATGAACCCAACTCCGGTCATGCCACAATTAGAGGTTCTCAATCGACTCGCGAACAACACGAAATTAATATCGGACCGCCCAAGGACTTTTCGTTCGATAAGAGACAAGCGGCTTCTTCCTCCTATAATATGCCCCTTGTTTTTCGGCTCACTGATCCAAATTCCATGTCAGAGAGTGAAGCCATGGTGATAGTGGAAGGATTGTTGGGGAAGCTGGCTCGTCGGTGCTATGCGGTGTTGTGTCTGGGGGTGCCGGTTGTGTCGGTGACGGTGGCTCACTTCATTAAAGGAACTATGGATCTGCCGGAGGAACCTACTCAACCTAGAGAGCGCAGCAAGCGCCGTCTTCGAGCTTTGCAGGGTCAGCTCTTCGTACCAATCGAGTCTAGCTACTGA

gene23134-vl.0-ab_initio

氨基酸序列：

MAALEYERTLEKTPTWAVAAVCFVLLVISIFIEHVINFIGKWFRSRQKRALYEALEKIKSELMLLGFLSLLLTVLQDPISGICIPKSVAATWHPCNDDPPKTTAGKLEEYSDSGFIFRRQLATKGYDKCTAHGKVAFVSAYGIHQLHIFIFVLAIFHVLYCITTLFLGRHKMRIWKYWENETKTIEYQFSTDPERFRYTRDTSFGKRHLHFWSESPVFLWIVCFFRQFYRSVTKTDYLTLRHGFITAHLAPGSETTFDFRKYISRSLEDDFLVIVEISPIIWFSAVLFLLSNAYGWHSYLWLPFISLIIILVVGTKLQVIITKLGLRIQDRGGVVMGAPLVQPGDQHFWFGRPRFLLFLIHFALFQNAFQLAFFAWSTWQFGITSCFHQTPQDTAIRLSVGVIVQVLCSYVTLPLYALVTQNANNRSEVTDGDDLFPPPDASVVVDMQHWDTEGSRSPKNNVGSSERGGIRQPSTFELGRTQELPAFSLSHPEVSSPSGISSARARK

核苷酸序列：

ATGGCGGCACTCGAATATGAGCGTACTTTAGAGAAAACTCCAACATGGGCAGTTGCTGCGGTCTGTTTTGTGTTGCTTGTAATATCAATTTTCATCGAGCATGTCATCAACTTCATAGGAAAGTGGTTTAGAAGTAGACAGAAGCGAGCTCTCTATGAAGCTCTTGAGAAGATCAAATCAGAGCTTATGCTGTTGGGATTCCTATCCTTGCTCCTAACAGTTCTACAAGATCCAATTTCTGGTATATGTATACCAAAGAGTGTTGCAGCCACTTGGCATCCTTGCAATGATGACCCTCCAAAGACTACAGCTGGGAAACTTGAGGAATACTCAGATTCTGGCTTCATTTTCCGGCGCCAATTAGCCACAAAAGGATATGATAAGTGCACGGCTCATGGTAAAGTTGCCTTTGTGTCTGCATATGGGATTCACCAGCTCCATATATTCATCTTTGTGCTAGCAATTTTTCATGTGCTCTACTGCATCACAACTTTGTTTTTGGGAAGACACAAGATGAGAATATGGAAGTATTGGGAGAATGAGACAAAAACAATTGAATACCAGTTTAGTACTGATCCAGAAAGGTTTAGGTATACGAGGGATACATCATTTGGAAAGAGGCATTTACACTTCTGGAGCGAGTCACCAGTTTTCCTTTGGATTGTATGCTTCTTCAGACAGTTCTACAGGTCAGTTACTAAGACTGATTACCTGACTCTGAGGCATGGCTTTATCACGGCACATTTAGCACCTGGGAGTGAAACGACCTTTGATTTTCGGAAGTACATAAGTAGATCACTTGAAGATGACTTCTTAGTTATTGTTGAGATAAGTCCAATTATATGGTTCTCAGCAGTGCTGTTCCTGTTGTCTAATGCATATGGATGGCATTCGTATCTCTGGCTACCATTTATCTCTTTAATTATAATCCTTGTGGTGGGAACTAAACTACAAGTGATTATAACCAAGCTGGGGCTAAGGATTCAGGATAGAGGGGGTGTGGTGATGGGTGCACCGCTTGTTCAACCCGGTGATCAGCACTTCTGGTTTGGACGCCCGCGCTTCTTGCTCTTCCTCATCCACTTTGCTTTATTCCAGAATGCATTTCAGCTGGCCTTCTTTGCATGGAGTACTTGGCAGTTTGGGATAACTTCTTGCTTCCATCAAACCCCTCAAGATACCGCCATCAGGCTCTCAGTGGGGGTTATCGTACAAGTTTTATGTAGTTATGTGACTCTGCCACTCTATGCTCTAGTGACACAGAATGCCAACAACCGCAGTGAGGTCACTGATGGGGATGACCTATTTCCACCTCCAGACGCATCAGTCGTAGTTGATATGCAACATTGGGACACTGAAGGGTCACGTTCCCCGAAGAACAATGTTGGATCGAGTGAAAGAGGAGGAATTAGGCAACCTAGTACTTTTGAGTTAGGCCGAATTCAAGAGCTACCTGCATTCTCCTTGTCTCACCCTGAAGTTAGTAGTCCCTCAGGCATCTCATCTGCTAGAGCAAGAAAGTGA。

2.权利要求1所述快速鉴定草莓白粉病基因的应用，包括：

1)携带有抗白粉病MLO基因的育种材料的创制或者新品种创制：利用权利1所给出的MLO基因源为亲本材料，在杂交、回交后代中，可以获得转育有MLO抗性基因的育种材料。

2)抗白粉病基础理论研究：对权利1的MLO型抗白粉病基因进行分子标记分析；对该基因进行分子作图或者基因定位；对该基因进行克隆以及基因间互作分析。

3)抗白粉病转基因研究：利用权利要求1所给出的MLO型白粉病基因进行转基因研究。