[发明专利]草鱼肠道组织腺苷酸基琥珀酸裂解酶基因序列

摘要

本发明公开了草鱼肠道组织腺苷酸基琥珀酸裂解酶基因。通过大规模的EST测序，得到草鱼肠道组织腺苷酸基琥珀酸裂解酶基因的部分序列，以此序列设计引物，应用RT-PCR技术和RACE技术克隆到草鱼肠道组织腺苷酸基琥珀酸裂解酶基因的全表达序列。对于研究草鱼体内的腺苷酸基琥珀酸裂解酶的基因结构组成，探讨其行使催化能力的功能区域位置及与其它动物的区别，从而对于研究其重组蛋白在生产中的应用提供重要的理论依据。

主权项

1. 草鱼肠道组织腺苷酸基琥珀酸裂解酶基因，其特征在于以下的RNA核苷酸及相应的氨基酸序列：aacagaaaac cgcgtgcagt cttgttttcg cggcactgag cgcttcgctg aactgctatt 60aac atg gag gga tcc ggg gac gag ttc atg aaa tac 96Met Glu Gly Ser Gly Asp Glu Phe Met Lys Tyr1 5 10cgc tca ccg ctg gtg tcc cgg tac gcc agc aaa gag 132Arg Ser Pro Leu Val Ser Arg Tyr Ala Ser Lys Glu15 20atg gcc tat aac ttc agc gac agg aaa aaa ttc aca 168Met Ala Tyr Asn Phe Ser Asp Arg Lys Lys Phe Thr25 30 35act tgg aga aaa ctg tgg att tat ctc gct aag gcc 204Thr Trp Arg Lys Leu Trp Ile Tyr Leu Ala Lys Ala40 45gag aag gca ttg ggt ctc ccc ata acc gat gcc cag 240Glu Lys Ala Leu Gly Leu Pro Ile Thr Asp Ala Gln50 55gtg agc gag atg gag tct cac gcg gag gac att gat 276Val Ser Glu Met Glu Ser His AIa Glu Asp Ile Asp60 65 70ttc gtc atg gct gca gag gaa gag agg aag ttg agg 312Phe Val Met Ala Ala Glu Glu Glu Arg Lys Leu Arg75 80cat gat gtc atg gct cat gtg cac acg ttc gct cat 348His Asp Val Met Ala His Val His Thr Phe Ala His85 90 95tgc tgt ccc act gct gct ccc atc atc cac ttg ggt 384Cys Cys Pro Thr Ala Ala Pro Ile Ile His Leu Gly100 105gcc acg tcc tgt tat gtg gga gac aac acg gat ctg 420Ala Thr Ser Cys Tyr Val Gly Asp Asn Thr Asp Leu110 115atc atg cta cgt gat gga ttt gat att ctg cta ccc 456Ile Met Leu Arg Asp Gly Phe Asp Ile Leu Leu Pro120 125 130aag ttg gct cgt gtc ata gac agg cag gct aac ttt 492Lys Leu Ala Arg Val Ile Asp Arg Gln Ala Asn Phe135 140gca gag aaa tat gct gac cag ccg act tta ggc ttc 522Ala Glu Lys Tyr Ala Asp Gln Pro Thr Leu Gly Phe145 150 155acc cac tat caa cca gct cag ctg act act gtc ggg 564Thr His Tyr Gln Pro Ala Gln Leu Thr Thr Val Gly160 165aag cgt gcg tgt ctg tgg ctg cag gat ctg gtc atg 600Lys Arg Ala Cys Leu Trp Leu Gln Asp Leu Val Met170 175gac atg cgc aat ctt cag cgt gca aga gag gac ctg 636Asp Met Arg Asn Leu Gln Arg Ala Arg Glu Asp Leu180 185 190cgc ttc agg ggg gtc aaa gga acc aca ggc act cag 672Arg Phe Arg Gly Val Lys Gly Thr Thr Gly Thr Gln195 200gcc agc tct ctg cag ctc ttc cag ggg gat cat gag 708Ala Ser Ser Leu Gln Leu Phe Gln Gly Asp His Glu205 210 215aag gtg gag gag ttg gat agg atg gtc act gag atg 744Lys Val Glu Glu Leu Asp Arg Met Val Thr Glu Met220 225gct gga ttt aaa aaa tca tac ttg gtg acg ggt cag 780Ala Gly Phe Lys Lys Ser Tyr Leu Val Thr Gly Gln230 235acg tac agt cgt aag gtg gat gtc gac tca ctc tgt 816Thr Tyr Ser Arg Lys Val Asp Val Asp Ser Leu cys240 245 250gtg ctg gcc agc ctc gga gcc aca gta cac aag att 852Val Leu Ala Ser Leu Gly Ala Thr Val His Lys Ile255 260tgc act gat att cgt ctg tta gct aat ctg aaa gag 888Cys Thr Asp Ile Arg Leu Leu Ala Asn Leu Lys Glu265 270 275atc gag gag cct ttt gag aaa gag cag att ggt tcc 924Ile Glu Glu Pro Phe Glu Lys Glu Gln Ile Gly Ser280 285agt gcc atg ccg tac aag aga aac ccc atg cgt gca 960Ser Ala Met Pro Tyr Lys Arg Asn Pro Met Arg Ala290 295gag cgc tgc tgt agt ctt gct cgc cac ctg atg gcg 996Glu Arg cys Cys Ser Leu Ala Arg His Leu Met Ala300 305 310ctg gtg tca gac cct ctg cag act gca tca gtg cag 1032Leu Val Ser Asp Pro Leu Gln Thr Ala Ser Val Gln315 320tgg ctg gaa aga acc cta gac gac agc gct aac agg 1068Trp Leu Glu Arg Thr Leu Asp Asp Ser Ala Asn Arg325 330 335agg atc tcg ttg ccc gag tcc ttc ctc aca gct gac 1104Arg Ile Ser Leu Pro Glu Ser Phe Leu Thr Ala Asp340 345atc atc ctc agc act ctg cag aac atc act gaa ggg 1140Ile Ile Leu Ser Thr Leu Gln Asn Ile Glu Gly350 355ctg gtg gtg tat cct aag gtg atc gag agg cat atc 1176Leu Val Val Tyr Pro Lys Val Ile Glu Arg His Ile360 365 370cgt cat gag ctg ccc ttc atg gct act gaa aac atc 1212Arg His Glu Leu Pro Phe Met Ala Thr Glu Asn Ile375 380atc atg gcc atg gtg aag gct gga gga aac aga cag 1248Ile Met Ala Met Val Lys Ala Gly Gly Asn Arg Gln385 390 395gac tgc cac gag aag atc cgt gtg ctg tcc cag caa 1284Asp cys His Glu Lys Ile Arg Val Leu Ser Gln Gln400 405gct gca gct gtg gtc aaa cag gaa ggg gga gat aat 1320Ala Ala Ala Val Val Lys Gln Glu Gly Gly Asp Asn410 415gac cta ctg gct cgg gtt cag gct gat cca tat ttc 1356Asp Leu Leu Ala Arg Val Gln Ala Asp Pro Tyr Phe420 425 430act ccc ata ctt gga cag ctg gac gcc tta ctg gat 1392Thr Pro Ile Leu Gly Gln Leu Asp Ala Leu Leu Asp435 440ccc aaa acc ttc atc ggc cga gcc cca caa cag gtt 1428Pro Lys Thr Phe Ile Gly Arg Ala Pro Gln Gln Val445 450 455aca agg ttt ttg tct gag gag gtc agg cct gtt cta 1464Thr Arg Phe Leu Ser Glu Glu Val Arg Pro Val Leu460 465gac cca tac aaa agt aaa atg gat gtg aag att gaa 1500Asp Pro Tyr Lys Ser Lys Met Asp Val Lys Ile Glu470 475cta gag ctt tgagacgtgc gactctgtga ctgatgcatc aaaacggtat 1549Leu Glu Leu480aacaatgaat aaatgtctag ctgtttaaaa aaaaaaaaaa 1689