[发明专利]家蚕微孢子虫极丝蛋白PTP3基因及用途

摘要

一种家蚕微孢子虫的繁殖和侵染有关的重要蛋白基因PTP3，依据家蚕微孢子虫的9倍基因组数据，得到编码家蚕微孢子虫极管蛋白PTP3基因序列CDS，并经一系列生物技术克隆证实。本发明所获得的极管蛋白PTP3对研究微孢子虫繁殖和侵染机制有其重要作用。从而对生产实践和微孢子虫病的防治发挥作用。RNAi干涉等试验分析表明，把极丝蛋白PTP3干涉到后，微孢子虫失去感染宿主的能力。所以微孢子虫的极丝蛋白PTP3和它潜在的调控基因将成为治疗微孢子虫感染新的因素。还可以通过转基因的手段，使之它高丰度的表达，从而杀害蝗虫等部分害虫。

主权项

1、一种家蚕微孢子虫极丝蛋白PTP3基因，其特征在于其序列如下：1 atgatctaca cattatttgt aatatacata tctagcacat tttgtaggat aatctcaaga61 agtacagtag aaaatggtcc tcccacagtt cctttcgatg aacatcaagc taaacatgtt121 catgttcgag tgcacggtag acacagacat ggaaatcacc atggtggtgg tcaagacgta181 cacgaagttc aaaaacacca cagtcatcac cacaatgggc ataattacgg tcttgaacat241 ggaaaattcg gaaatgtaac ttacacttct ggaaatcacc atggtcacca ttctcatcac301 ggttctcacg cggatggaca ccattctcaa tacggttcgc acgcggacgg acatcatgtt361 aacgcccaat cagatgcttc tgcaccacta gacgttttgc caatgaaaga agtaagaagt421 ggtgatgatc cagctttgga gagagatcta ggaattcaac aagccatgaa agcttttcga481 tcttctttac ctgtttcaaa taaagacacc aattctcttt tggataaagc tgaaggaatt541 ttaagaagtg caccagacgg ccaattagat gctcctgttc ttgatagtta cggaaatcca601 actgggcaga catacagaga agccgcagaa gatgcagtag gctataagta tccaacagca661 gtagcagatg aagaattcga tagtttagta aggagtggtg ttgaaccggg aagagccaag721 caagtagctg ataaaattga agaaacaggt tttgaacacg agaaaattaa agatgcagta781 agaaatgaag tagctagagg tacagcaaac agagccgtag cagaattaca agacgaaatt841 caaagacgta aaaatgctac taaagtcaaa gaacgtgtta aagttgaaga tgaattgtac901 agacaacaca ctcttcctgc tactggttta ttcgatgaag acgtgtctgt tctgaaaaat961 atgagccaaa ttgttccgcc tgcgaaaatt gtcgactaca tcagagactc ctatgataat1021 tcaattgaag ccggacttcc tcctgcggtt gctcgtgctc aggccgatcg tgttggttct1081 ttaattacgg atttcaatac aatgttaaga agtggtgaat ctcctgaaac tgctttgtcc1141 catcttaaag acatgtctga taggcaatca aaagtcgatc aaagaagaga attacttcag1201 aactctagtc cagaagtctt aaaaatactt tcagataaag ataatttaat taaagaagca1261 ctggttaaaa ctgcaagaga tcctgactgt agagacagat actttgatga agcctcccag1321 aagatgaaga ttcttgatgc aagagaagcc agttttattc atgatactga aaataacaag1381 cacatacgtc gtcctaggaa aatggcagaa gatttaatgg ataagactaa cagagataat1441 gcagaattag ttgatgtagc ttatagtcac ccagtagtag ccaaagacat tttaaaacaa1501 ggtattgcaa acactgaaag agtagctgag aaattggctg atcatttgga tgctgtaaaa1561 tttgctgaaa aagctcaaaa tccttcagct gtttcggctt tgaaacttct tgacgagaga1621 aagagacaag atgaacttga aaaaatcaat aagagaaata cagaaaatga gcaaaagatg1681 ttcaagcaga ttctaaaaga ccaagaagac gcggaagtaa tgcaaaccga agagaaaatg1741 gcagccgcaa acaaagcaag aaagatttta gatgaaggac gtagggcagc tgaacaagtt1801 ttgaaagcgg gtggaagtac tgatgaagct gctgctgttc gtgttgcagc tgagaatgct1861 actgctcgag gtttggaaca aagagaatcc atgatcgaag aagagattat gaaaaggttt1921 ggaggcgcag gtgatcctga aaaagctcgt aaaatggctg ctcacattgc tatcgctcac1981 agtggagaaa atgccgtagg aaaaacgccc gaagaagtca ttaagaaaga acaagaagcg2041 caaaagaaag agattttaag aaatgctttg tatgatgata ttggaaaaga accaatcgaa2101 attaagtctg taattccaga agaccgagat gaatactcta ttgacagaag tgtaattaaa2161 tttcctctta aaacatttat tgatgaagaa gtaagcaatg ttggagaagc ctacgtaaag2221 aacaaaaagc aaagtatcaa cgacgaagtc aggaataaag tcactaccac caacgtcaac2281 agcggtctta atgtaattga aacagaaaat ggtttcttga atctaggaga gaatagtaag2341 aaaatccaca ttgacgaagc cacagcctat tttaaaccgg ccgccaaatt aaaaatggag2401 aaaaaaggta ttaagacgga taatttcaga ccaaaaacca atcaaggtga aataagagac2461 atgcccaaag gagaaactta ctatgaggtt gatcttaaag acgccgatct aagtcttcct2521 tctcctacta atcctactcc tgatacactt gtctcaaatg gaaaagacgt tgttgatgtt2581 gaagatgttt ctgccattgt aattaataaa ggtactttag tccagacacc atggaacgaa2641 tactctgaca tgattaagcc gaagtttaat ccgtatccaa atgaaggaga gaaaattaat2701 caaatcaaga aacttcaaaa actcatagcc gatgagaaga gaaaagacac attagaccga2761 attaaagtta acacaattac taatcctgac ggagaaaagc gaatgattgt taatacgcct2821 tatggtgttc aagaaatgtt cgaggaaact gaaggaatga aaagactttc tcatattgat2881 ccaaataaga acgtggctat tagtgaaact ccgactgatg ataacaaaga atctatttac2941 agcgccttta agaacgtttc accaaatgaa gcagtcggaa aattcattga agatactttt3001 aattctgcat actcatccgg tcaagctcag agatttgtaa atcccacaaa cgcctttact3061 ggtcaagaag atccaaagaa agccagactc atgactcaga agactattga tgttacagag3121 tattatatta atgacggtaa accatcttcg gctatcaaaa atcaactaat ccaaaattac3181 agagccttag ctgatagcct tggaatgacc gaagaagatt tcattcaatt cgctcgtaaa3241 attcctgatg attctttggc acaaatgatc tcttataatg aacaatcaga atcttcgagg3301 cccgcattag tcgatgctcc attctttaag acacttcagc cacttgcaaa tcaaacgtca3361 aagaccaaac ttaacgacat catcaaaata atcttcacac aaatttctaa agtaactggt3421 aaaaccaatt caaccacagg cacaacactc gagaagaaga tagttcctaa tctaaaggca3481 gtaagatctg accaagtcat tgcaggacca aatggaggaa cttcagcttt atcccaagcc3541 acagctccaa gaaccggttc tacagttttg agtaagcaaa tcacacgagg acttcccaga3601 gttccttcag gtacaggtac tagttatcca gtgagagatc ccaatggtat taatacaagt3661 gaagataatg aaagatataa agtcaatcct tataatccat attctaaatc tgatactagt3721 ggtcttgaag cacctggtgg tgaaatagta cataatggta caagaccaag tccttacgct3781 atagtaggag ttccaatagt cgcagcggtt acaacaccag tcatcagacc agcagtctta3841 agaacgcctc cagcagcaca atcgagttct agtgcacttc aaggaaatac agcacttact3901 ggagcaggaa caccaagagc cggagtagca ggatcaccag gagtaaatcc aggaccaact3961 ggtaaagcac ctgtctcacc ccctgcacca acatcaccag cttcatctcc tacagcctca4021 cctatagcac ctacagcttc acctatagca cctacaggag catcaatagg agcacctggt4081 tcacttggaa tcccatcatc accattgaag。