[发明专利]一种稳定、抗干扰能力强的直接胆红素（氧化酶法）检测试剂及检测方法

摘要

本发明涉及血清直接胆红素检测技术领域，特别涉及一种血清直接胆红素(氧化酶法)检测试剂，试剂R1中含有缓冲液，氟化钠，NAC(N‑乙酰半胱氨酸)，4‑甲苯磺酸钠，脂肪酶，抗坏血酸氧化酶，α‑环糊精，蔗糖，海藻糖，PEG‑6000，脂肪醇聚氧乙烯醚(AEO‑9)，防腐剂；试剂R2中含有缓冲液，胆红素氧化酶，蔗糖，海藻糖，PEG‑6000，脂肪醇聚氧乙烯醚(AEO‑9)，防腐剂。本试剂明显提高了测试直接胆红素时的特异性和准确性，可防止反应体系浑浊、促进反应进行，使试剂的稳定性、抗干扰能力进一步提高。

主权项

1.一种血清直接胆红素（氧化酶法）检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1中含有缓冲液_{························································}50mmol/L，氟化钠_{························································}2 mmol/L，NAC（N‑乙酰半胱氨酸）_{···········································}0.2 mmol/L，4‑甲苯磺酸钠_{··················································}40 mmol/L，脂肪酶_{·······^·················································}15KU/L，抗坏血酸氧化酶_{···············^·································}8 KU/L，α‑环糊精_{······················································}1.5g/L，蔗糖_{··························································}40g/L，海藻糖_{························································}10g/L，PEG‑6000_{······················································}2g/L，脂肪醇聚氧乙烯醚（AEO‑9）_{·······································}1g/L，防腐剂_{························································}0.2mL/L；2）试剂R2的组分为：缓冲液_{························································}50mmol/L，胆红素氧化酶_{··················································}12KU/L，蔗糖_{··························································}40g/L，海藻糖_{························································}10g/L，PEG‑6000_{······················································}2g/L，脂肪醇聚氧乙烯醚（AEO‑9）_{·····^··································}1g/L，防腐剂_{························································}0.2mL/L。