[发明专利]一种稳定、抗干扰能力强的α-羟丁酸脱氢酶试剂及检测方法

摘要

本发明涉及α‑羟丁酸脱氢酶检测技术领域，特别涉及一种α‑羟丁酸脱氢酶检测试剂，试剂R1中含有缓冲液，乙二胺四乙酸二钠(EDTA‑2Na),α‑酮丁酸,十二烷基二甲基甜菜碱，防腐剂；试剂R2中含有缓冲液，NADH，BSA，蔗糖，海藻糖，甘露醇，十二烷基二甲基甜菜碱，防腐剂。采用PIPES（哌嗪‑1,4‑二乙磺酸）缓冲液，添加稳定剂BSA,蔗糖，海藻糖，甘露醇，大大增强了试剂的稳定性；采用两性表面活性剂十二烷基二甲基甜菜碱，不仅显著改善测定的性能，而且增强了试剂的稳定性和抗干扰能力。

主权项

1.一种α‑羟丁酸脱氢酶检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1中含有哌嗪‑1,4‑二乙磺酸缓冲液，25℃，pH为7.4··························50mmol/L，乙二胺四乙酸二钠·········· ·········3mmol/L，α‑酮丁酸········· ··············5mmol/L，十二烷基二甲基甜菜碱·· ···············2g/L，NaN₃··············· ··········0.5g/L；2)试剂R2的组分为：哌嗪‑1,4‑二乙磺酸缓冲液，25℃，pH为7.4·········50mmol/L,NADH·························0.3mmol/L，BSA············ ·············1g/L，蔗糖·························10g/L，海藻糖························3g/L，甘露醇························10g/L，十二烷基二甲基甜菜碱··· ··········· ··2g/LNaN₃·········· ···············0.5g/L；R1试剂和R2试剂的比例为4:1。