[发明专利]配子体花粉自交不亲和性基因及其应用

摘要

本发明提供了一种金鱼草S基因，该基因具有说明书所述的核苷酸序列。本发明还提供了使用该基因鉴定金鱼草植株之间自交亲和性的方法、用于鉴定金鱼草植株之间自交亲和性的PCR引物对、以及一种改变金鱼草自交不亲和性的方法。

主权项

1.一种金鱼草S基因，它具有以下的核苷酸序列：1 gagaactagg tttgagattt atccaaaatc aaagacttag ttaggcagta51 tatatataga aaattgagac ttttcaccaa tgaaagactt agtctaattt101 ttttttcttt tacgagtgaa aaatatacgt atttctttta ttaaacttat151 tcaacatgat taaacatttt tacttttgtc accatatgac atcaatttat201 ttatatctga acagtatacg tggtaagtat aaaggtccga actgaaatcc251 gcacacttta tacctaaatt agacaaagcg gaagattata cgttctcttt301 ctaaaatttg caaatcatgc aaagtggacg cgtataccat ctatccgcat351 cgaccataca gtatctccac aattttggtt gacccattcc ttccgcagcg401 ttttgttaat ctctttagct ttcattgatt cttcctcata tgctcgatcc451 gtcaatacat atttctacta ctattttttg gtttataaca acaatataaa501 aattttcaaa tggcctttaa tcttattcga tagttcgtgt aattaagacg551 tacgaaggcc cactagacaa cgataaggtt ccgtgggcaa tattaggcgt601 ggaaaatgat ggatcgacga tttccgcgac aggatgtaat tagtgaaatt651 ttgttattct cgtcagtgaa atccctgcta aggttcaggt gcgtctcaaa701 gtcttggtgt tctctaatca aaagtaatga cttcatagat aatcatctcc751 taagacgaca aacaaatggt aatgtaatgg ttgtaaagcg gtatgtacgg801 acaccagaaa gggatatgtt ttccttctac aatatcaatt ctccagaact851 agacgaattg ttgccagatc tacccaatcc atatttcaag aatataaaat901 tcgactacga ttatttttac ttacctcaac gtgtcaacct aatgggccca951 tgcaacgggc tcatctgctt agcatatggt gattgcgttc ttttgtccaa1001 tcctgcactg cgagaaatca agcggcttcc acctacgcct tttgctaacc1051 cagagggtca ctgcaccgac attataggat acggatttgg taatacttgt1101 aacgattgtt acaaagttgt cttgatagag agtgtcgggc ctgaggatca1151 tcatatcaac atatacgtgt actattcaga caccaactcc tggaaacata1201 tcgaggatga ttctacacct attaagtaca tttgtcactt tccttgtaac1251 gagctcttct tcaaaggtgc tttccactgg aacgcaaact cgactgatat1301 tttttatgca gattttatcc ttacttttga tataatcacg gaggttttta1351 aggagatggc gtaccctcat tgtttagcgc aattctctaa cagtttttta1401 tctctcatgt ccttgaatga atgccttgct atggttcgat ataaagaatg1451 gatggaggat ccggaattat ttgacatttg ggttatgaat caatatggcg1501 tgcgtgagtc ttggaccaaa cagtatgtca taggacccca ggtagtcgta1551 tgctctcacg tatgctggaa gaatgacgag tgcctgatag ttgaagacgg1601 caatggccag ttagtatcct gtgcatttcg cacaaataag attgagaaac1651 ttccaatcta tgcagttgaa gaaacattga gagttctaat tgttgatgag1701 agcttaatca gtttgaacag ggtactaaat ttgtagaaca aaatacggtg1751 ccaaaaatat attacagagg catatatcta gaatttcgct catgaactat1801 gacatccatg atagtttttt tattctgcta agaaatttgt gtagtttagt1851 ttttcttata agtgtgggtt gcctcttgtg catctcctat atactgggaa1901 gcctgttatg cgaaaaggga gtgcccttac gttgttgaaa aaaactgttg1951 ttcacttctg acaacaaatt taaaatgtct agattc。